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For a 3 mg sermorelin vial, the standard volume is 2 mL, drawn in two 1 mL pulls if using a 1 mL syringe

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The next day, fixed tissues were washed three times in Dulbecco’s phosphate buffered saline (DPBS, GIBCO, #14190-094), and depending on the purpose, either embedded, frozen in optimal cutting temperature (OCT) compound (Sakura, #4583) and stored at –20 °C, or stored at 4 °C in PBS containing 0.2% gelatin (Sigma Aldrich, #G1393), 0.5% Triton X-100 (Sigma Aldrich, #X100) and 0.01% Thimerosal (Sigma Aldrich, #T8784) (PBS-GT)